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Introduction to Protein Electrophoresis

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

Abstract

This chapter discusses, briefly, the developments in electrophoresis of proteins from Tiselius’ moving boundary electrophoresis to the modern day two-dimensional polyacrylamide gel electrophoresis. It also touches upon the staining methods used to visualize total proteins postelectrophoresis.

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References

  1. Tiselius A (1938) Elektrophoretische Messungen am Eiweiss. Kolloid Z 85:129

    Article  CAS  Google Scholar 

  2. Righetti PG (2005) Electrophoresis: the march of pennies, the march of dimes. J Chromatogr A 1079:24–40

    Article  PubMed  CAS  Google Scholar 

  3. Von Klobusitzky P, Konig P (1939) Biochemische studien uber die gifte der schlan gengattung. Arch Exp Path Pharmakol 192:271–275

    Article  CAS  Google Scholar 

  4. Evered DF (1959) Ionophoresis of acidic and basic amino acids on filter paper using low voltages. Biochim Biophys Acta 36:14–19

    Article  PubMed  CAS  Google Scholar 

  5. Ingram VM (1958) The comparison of normal human and sicke-cell haemoglobins by fingerprinting. Biochim Biophys Acta 28:539–545

    Article  PubMed  CAS  Google Scholar 

  6. Smithies O (1955) Zone electrophoresis in starch gels: group variations in serum proteins of normal human adults. Biochem J 61:629–641

    PubMed  CAS  Google Scholar 

  7. Poulik MD (1957) Starch gel electrophoresis in a discontinuous system of buffers. Nature 180:1477–1479

    Article  PubMed  CAS  Google Scholar 

  8. Raymond S, Weintraub L (1959) Acrylamide gel as a supporting medium for zone electrophoresis. Science 1959(130):711–712

    Article  Google Scholar 

  9. Raymond S (1964) Acrylamide gel electrophoresis. Ann N Y Acad Sci 121:35–65

    Google Scholar 

  10. Ornstein L (1964) Disc electrophoresis. I. Background and theory. Ann N Y Acad Sci 28:32–49

    Google Scholar 

  11. Gelfi C, Righetti PG (1981) Polymerization kinetics of polyacrylamide gels I. Effect of different cross-linkers. Electrophoresis 2:213–219

    Article  CAS  Google Scholar 

  12. Righetti PG, Gelfi C, Bosisio AB (1981) Polymerization kinetics of polyacrylamide gels III. Effect of catalysis. Electrophoresis 2: 291–295

    Article  CAS  Google Scholar 

  13. Andrews AT (1986) Polyacrylamide gel electrophoresis (PAGE): homogeneous gel and buffer systems. In: Electrophoresis: theory, techniques, and biochemical and clinical applications. Oxford University Press, New York

    Google Scholar 

  14. Maurer HR (1971) Disc electrophoresis and related techniques of polyacrylamide gel electrophoresis, 2nd edn. Walter de Gruyter, Berlin

    Google Scholar 

  15. Margolis J, Kenrick KG (1967) Polyacrylamide gel-electrophoresis across a molecular sieve gradient. Nature 214:1334–1336

    Article  PubMed  CAS  Google Scholar 

  16. Margolis J, Kenrick KG (1967) Electrophoresis in polyacrylamide concentration gradient. Biochem Biophys Res Commun 27:68–73

    Article  PubMed  CAS  Google Scholar 

  17. Shapiro AL, Vinuela E, Maizel JV (1967) Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun 28: 815–820

    Article  PubMed  CAS  Google Scholar 

  18. Ornstein L (1964) Disc electrophoresis. I. Background and theory. Ann N Y Acad Sci 121:321–349

    Article  PubMed  CAS  Google Scholar 

  19. Davis BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci 121:404–427

    Article  PubMed  CAS  Google Scholar 

  20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  21. Steinberg TH (2009) Protein gel staining methods: an introduction and overview. Methods Enzymol 463:541–563

    Article  PubMed  CAS  Google Scholar 

  22. Neuhoff V, Stamm R, Hansjorg E (1985) Clear background and highly sensitive protein staining with coomassie blue dyes in polyacrylamide gels: a systematic analysis. Electrophoresis 6:427–488

    Article  CAS  Google Scholar 

  23. Switzer RC, Merril CR, Shifrin S (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98:231–237

    Article  PubMed  CAS  Google Scholar 

  24. Daban J-R, Bartolome S, Samso M (1991) Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 199:169–174

    Article  PubMed  CAS  Google Scholar 

  25. Berggren K, Chernokalskaya E, Steinberg TH et al (2000) Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex. Electrophoresis 21:2509–2521

    Article  PubMed  CAS  Google Scholar 

  26. Mackintosh JA, Choi H-Y, Bae S-H et al (2003) A fluorescent natural product for ultrasensitive detection of proteins in one-dimensional and two-dimensional gel electrophoresis. Proteom­ics 3:2273–2288

    Article  PubMed  CAS  Google Scholar 

  27. Steinberg TH, Agnew BJ, Gee KR et al (2003) Global quantitative phosphoprotein analysis using multiplexed proteomics technology. Proteomics 3:1128–1144

    Article  PubMed  CAS  Google Scholar 

  28. Kinoshita E, Kinoshita-Kikuta E, Takiyama K, Koike T (2006) Phosphate binding tag, a new tool to visualize phosphorylated proteins. Mol Cell Proteomics 5:749–757

    PubMed  CAS  Google Scholar 

  29. O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda, Bethesda, MD, USA

    Pothur R. Srinivas

Authors
  1. Pothur R. Srinivas
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Correspondence to Pothur R. Srinivas .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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Srinivas, P.R. (2012). Introduction to Protein Electrophoresis. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_2

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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