Abstract
The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
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Acknowledgments
This work was supported by AGL2008-05108-C03-01 (Ministerio de Ciencia e Innovación, Spain) and CSD2007-00063 FUN-C-FOOD projects (Programa CONSOLIDER, Ministerio de Educacion y Ciencia, Spain). MC thanks The Council of Higher Education, Turkey for his grant. CI thanks Ministerio de Educacion y Ciencia, Spain for her FPI grant.
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Çelebier, M., Ibáñez, C., Simó, C., Cifuentes, A. (2012). A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_15
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DOI: https://doi.org/10.1007/978-1-61779-821-4_15
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