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A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

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Abstract

Free flow electrophoresis (FFE) has been applied in numerous studies as a protein separation technique due to its multiple advantages such as fast and efficient sample recovery, high resolving power, high reproducibility, and wide applicability to protein classes. As a stand-alone platform however, its utility in comparative proteomic analysis is limited as protein samples must be run sequentially rather than simultaneously which introduces inherent variability when attempting to perform quantitative analysis. Here we describe an approach combining fluorescent CyDye technology (DIGE) with FFE to simultaneously separate and identify differentially expressed proteins in a model cell system.

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Author information

Authors and Affiliations

  1. CSIRO, Preventative Health Flagship, Australia, Adelaide BC, SA, Australia

    Kim Y. C. Fung

  2. CSIRO, Food and Nutritional Sciences, Adelaide BC, SA, Australia

    Kim Y. C. Fung, Tanya Lewanowitsch & Leah Cosgrove

  3. School of Molecular and Biomedical Science, Adelaide Proteomics Centre, University of Adelaide, Adelaide, Australia

    Chris Cursaro

  4. CSIRO, Preventative Health Flagship, Adelaide BC, SA, Australia

    Tanya Lewanowitsch & Leah Cosgrove

  5. School of Molecular and Biomedical Science, Adelaide Proteomics Centre, University of Adelaide, Adelaide BC, SA, Australia

    Peter Hoffmann

Authors
  1. Kim Y. C. Fung
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  2. Chris Cursaro
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  3. Tanya Lewanowitsch
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  4. Leah Cosgrove
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  5. Peter Hoffmann
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Corresponding author

Correspondence to Kim Y. C. Fung .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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© 2012 Springer Science+Business Media, LLC

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Fung, K.Y.C., Cursaro, C., Lewanowitsch, T., Cosgrove, L., Hoffmann, P. (2012). A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_12

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_12

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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