Abstract
As a result of the progress in sequencing technology, many plant genomes have now been determined. Functional genomics is required to clarify gene function in many of these species. To identify useful genes easily and quickly, we have developed a FOX (full-length cDNA overexpressor) hunting system in which full-length cDNAs are overexpressed in Arabidopsis plants. This system was applied to high-throughput analysis of rice genes through heterologous expression in Arabidopsis (rice FOX Arabidopsis lines). We demonstrated that it is possible to carry out high-throughput analysis of gene function by utilizing rice FOX Arabidopsis lines. In this protocol, we describe how to isolate candidate rice FOX Arabidopsis lines and to determine the rice fl-cDNA that is responsible for the observed phenotype.
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Acknowledgments
This work is supported by the Special Coordination Fund for Promoting Science and Technology awarded to M.M., K.O., and H.H. This study is also supported by the Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports and Technology of Japan (19710055) to Y.K and (21780315) to M.H. The rice transformation method described in this protocol was developed by Dr. Seiichi Toki and Dr. Hiroshi Tanaka (National Institute of Agrobiological Sciences). We thank Dr. Hirofumi Kuroda, Ms. Yoko Horii, and Dr. Yuko Tsumoto (RIKEN Plant Science Center) for technical support. We appreciate the helpful discussions with Dr. Takeshi Yoshizumi (RIKEN Plant Science Center).
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Higuchi, M., Kondou, Y., Mori, M., Ichikawa, T., Matsui, M. (2012). Characterization of Rice Genes Using a Heterologous Full-Length cDNA Expression System. In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_8
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DOI: https://doi.org/10.1007/978-1-61779-558-9_8
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