Abstract
Transgenic oil palm (Elaeis guineensis Jacq.) plantlets are regenerated after Agrobacterium tumefaciens-mediated transformation of embryogenic calli derived from young leaves of oil palm. The calli are transformed with an Agrobacterium strain, LBA4404, harboring the plasmid pUBA, which carries a selectable marker gene (bar) for resistance to the herbicide Basta and is driven by a maize ubiquitin promoter. Modifications of the transformation method, treatment of the target tissues using acetosyringone, exposure to a plasmolysis medium, and physical injury via biolistics are applied. The main reasons for such modifications are to activate the bacterial virulence system and, subsequently, to increase the transformation efficiency. Transgenic oil palm cells are selected and regenerated on a medium containing herbicide Basta. Molecular analyses revealed the presence and integration of the introduced bar gene into the genome of the transformants.
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Acknowledgments
The authors wish to thank the Director General of MPOB for permission to publish this paper. The authors would also like to thank Madam Hamidah Ghazali from MARDI for providing the pUBA vector. Special appreciation is accorded to Dr. Ahmad Tarmizi b. Hashim and Madam Esther Leela a/p Amirtham (Clonal Propagation Group, MPOB) for the supply of oil palm calli. Finally, the authors would like to acknowledge all staff of the Genetic Transformation Laboratory of MPOB for their assistance.
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Izawati, A.M.D., Parveez, G.K.A., Masani, M.Y.A. (2012). Transformation of Oil Palm Using Agrobacterium tumefaciens . In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_15
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DOI: https://doi.org/10.1007/978-1-61779-558-9_15
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