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Enrichment of Unstable Non-coding RNAs and Their Genome-Wide Identification

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Yeast Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 759))

Abstract

Cryptic unstable transcripts (CUTs) have been recently described as a major class of non-coding RNAs. These transcripts are, however, extremely unstable in normal cells and their analyzes pose specific technical problems. In this chapter, after a brief introduction discussing general aspects associated with the analysis of non-coding RNAs, we provide details of methods to enrich, map, and quantify this unconventional class of transcripts.

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Acknowledgments

We thank C. Saveanu, F. Feuerbach and M. Fromont-Racine for stimulating discussions and the staff of the Genoscope (Evry) for sequencing. This work was supported by the Institut Pasteur, the CNRS, the ANR (CUT program) and the European Science Foundation (RNA quality program). H.N. is supported by the ANR (CUT program).

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Authors and Affiliations

  1. Unité de Génétique des Interactions Macromoléculaires, Institut Pasteur, CNRS, URA2171, Paris, France

    Helen Neil & Alain Jacquier

Authors
  1. Helen Neil
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  2. Alain Jacquier
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Correspondence to Alain Jacquier .

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Editors and Affiliations

  1. , Department of Biochemistry, University of Cambridge, Tennis Court Road 80, Cambridge, CB2 1GA, United Kingdom

    Juan I. Castrillo

  2. , Department of Biochemistry, University of Cambridge, Tennis Court Road 80, Cambridge, CB2 1GA, United Kingdom

    Stephen G. Oliver

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© 2011 Humana Press

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Neil, H., Jacquier, A. (2011). Enrichment of Unstable Non-coding RNAs and Their Genome-Wide Identification. In: Castrillo, J., Oliver, S. (eds) Yeast Systems Biology. Methods in Molecular Biology, vol 759. Humana Press. https://doi.org/10.1007/978-1-61779-173-4_6

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  • DOI: https://doi.org/10.1007/978-1-61779-173-4_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-172-7

  • Online ISBN: 978-1-61779-173-4

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