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Fluorescence Fluctuation Spectroscopy and Imaging Methods for Examination of Dynamic Protein Interactions in Yeast

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Yeast Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 759))

Abstract

Protein interactions are inherently dynamic. In no system is this more true and important than in signaling pathways, where spatial and temporal control of specific protein interactions is key to signaling specificity and timing. While genetic and biochemical interactions form a necessary and important starting point for deciphering interactions among signaling components, they struggle to provide precise information of where and when interactions occur in a live cell setting. In contrast, live cell fluorescence studies such as those outlined below are able to provide quantitative information on the strength, nature, timing, and location of homotypic and heterotypic protein interactions.

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Acknowledgments

We thank Veronica Conaway (Stowers Institute for Medical Research, SIMR) for discussion on yeast medium, Winfried Wiegraebe (SIMR) for discussions on fluctuation spectroscopy, and Arupratan Das (SIMR) for critical reading of the manuscript.

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Authors and Affiliations

  1. Stowers Institute for Medical Research, Kansas City, MO, USA

    Brian D. Slaughter, Jay R. Unruh & Rong Li

  2. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA

    Rong Li

Authors
  1. Brian D. Slaughter
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  2. Jay R. Unruh
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  3. Rong Li
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Corresponding author

Correspondence to Brian D. Slaughter .

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Editors and Affiliations

  1. , Department of Biochemistry, University of Cambridge, Tennis Court Road 80, Cambridge, CB2 1GA, United Kingdom

    Juan I. Castrillo

  2. , Department of Biochemistry, University of Cambridge, Tennis Court Road 80, Cambridge, CB2 1GA, United Kingdom

    Stephen G. Oliver

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© 2011 Humana Press

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Slaughter, B.D., Unruh, J.R., Li, R. (2011). Fluorescence Fluctuation Spectroscopy and Imaging Methods for Examination of Dynamic Protein Interactions in Yeast. In: Castrillo, J., Oliver, S. (eds) Yeast Systems Biology. Methods in Molecular Biology, vol 759. Humana Press. https://doi.org/10.1007/978-1-61779-173-4_17

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  • DOI: https://doi.org/10.1007/978-1-61779-173-4_17

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  • Print ISBN: 978-1-61779-172-7

  • Online ISBN: 978-1-61779-173-4

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