Abstract
Gene transcription is a dynamic process in which the desired amount of an mRNA is obtained by the equilibrium between its transcription (TR) and degradation (DR) rates. The control mechanism at the RNA polymerase level primarily causes changes in TR. Despite their importance, TRs have been rarely measured. In the yeast Saccharomyces cerevisiae, we have implemented two techniques to evaluate TRs: run-on and chromatin immunoprecipitation of RNA polymerase II. These techniques allow the discrimination of the relative importance of TR and DR in gene regulation for the first time in a eukaryote.
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Acknowledgments
We wish to thank Priyanka Palit, Toni Jordán, and Fany Carrasco for their help in optimizing the GRO protocol, and also Sebastián Chávez and Paula Alepuz for critically reviewing the manuscript. This work has been supported by grants BFU2007-67575-CO3-01/BMC from the Spanish Ministry of Education and Science and by grant ACOMP/2009/368 from the Generalitat Valenciana (Valencian Regional Government) awarded to JEP-O.
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García-Martínez, J., Pelechano, V., Pérez-Ortín, J.E. (2011). Genomic-Wide Methods to Evaluate Transcription Rates in Yeast. In: Becskei, A. (eds) Yeast Genetic Networks. Methods in Molecular Biology, vol 734. Humana Press. https://doi.org/10.1007/978-1-61779-086-7_2
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DOI: https://doi.org/10.1007/978-1-61779-086-7_2
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