Skip to main content
SpringerLink
Log in

Muskmelon Embryo Rescue Techniques Using In Vitro Embryo Culture

  • Protocol
  • First Online:
Plant Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 710))

Abstract

Among the major cucurbit vegetables, melon (Cucumis melo) has one of the greatest polymorphic fruit types and botanical varieties. Some melon fruits have excellent aroma, variety of flesh colors, deeper flavor, and more juice compared to other cucurbits. Despite numerous available melon cultivars, some of them are exceedingly susceptible to several diseases. The genetic background carrying the genes for tolerance and/or resistance for those diseases is found in wild melon landraces. Unfortunately, the commercial melon varieties are not able to produce viable hybrids when crossed with their wild melon counterparts. Plant tissue culture techniques are needed to surpass those genetic barriers. In vitro melon embryo rescue has played a main role to obtain viable hybrids originated from commercial versus wild melon crosses. In this chapter, an efficient and simple embryo rescue melon protocol is thoroughly described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Food and Agriculture Organization (FAO) (2007) FAOSTAT data. http://www.fao.org/corp/statistics/en/. Retrieved 3 March 2009

  2. Zitter TA, Hopkins DL, Thomas CE (1998) Compendium of cucurbit diseases. APS, St. Paul, MN

    Google Scholar 

  3. Fehr WR, Fehr EL, Jessen HJ (1987) Principles of cultivar development, vol 1, Theory and technique. Collier Macmillan, New York

    Google Scholar 

  4. Guillaume R, Boissot N (2001) Resistance to Diaphania hyalinata (Lepidoptera: Crambidae) in Cucumis species. J Econ Entomol 94:719–723

    Article  PubMed  CAS  Google Scholar 

  5. De Dias CS, Pico B, Espinos A, Nuez F (2004) Resistance to melon vine decline derived from Cucumis melo ssp. agrestis: genetic analysis of root structure and root response. Plant Breed 123:66–72

    Article  Google Scholar 

  6. Martinez L, Agüero CB, Galmarini CR (1997) Obtention of haploid plants by ovaries and ovules culture in onion (Allium cepa L.). Acta Hortic 433:447–454

    Google Scholar 

  7. Xynias IN, Zamani IA, Gouli-Vavdinoudi E, Roupakias DG (2001) Effect of cold pretreatment and incubation temperature on bread wheat (Triticum aestivum L.) anther culture. Cereal Res Commun 29:331–338

    Google Scholar 

  8. Weymann K, Urban K, Ellis DM, Novitzky R, Dunder E, Jayne S, Pace G (1993) Isolation of transgenic progeny of maize by embryo rescue under selective conditions. In Vitro Cell Dev Biol 29P:33–37

    Google Scholar 

  9. Eijlander R, Stiekema WJ (1994) Biological containment of potato (Solanum tuberosum) – outcrossing to the related wild-species black nightshade (Solanum nigrum) and bittersweet (Solanum dulcamara). Sex Plant Reprod 7:29–40

    Article  Google Scholar 

  10. Faure N, Serieys H, Kaan F, Berville A (2002) Partial hybridization in crosses between cultivated sunflower and the perennial Helianthus mollis: effect of in vitro culture compared to natural crosses. Plant Cell Rep 20:943–947

    Article  CAS  Google Scholar 

  11. Alemanno L, Guiderdoni E (1994) Increased doubled haploid plant-regeneration from rice (Oryza sativa L.) anthers cultured on colchicine-supplemented media. Plant Cell Rep 13:432–436

    Article  CAS  Google Scholar 

  12. Hoekstra S, Vanzijderveld MH, Louwerse JD, Heidekamp F, Vandermark F (1992) Anther and microspore culture of Hordeum vulgare L. cv Igri. Plant Sci 86:89–96

    Article  CAS  Google Scholar 

  13. Lotfi M, Alan AR, Henning MJ, Jahn MM, Earle ED (2003) Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance. Plant Cell Rep 21:1121–1128

    Article  PubMed  CAS  Google Scholar 

  14. Marcellan ON, Camadro EL (2000) Preliminary results on embryo rescue for ­circumventing hybridization barriers in Asparagus. Biocell 24:247–251

    PubMed  CAS  Google Scholar 

  15. de Oliveira ACB, Maluf WR, Pinto JEBP, Azevedo SM (2003) Resistance to papaya ringspot virus in summer squash Cucurbita pepo L. introgressed from an interspecific C. pepo x C. moschata cross. Euphytica 132:211–215

    Article  Google Scholar 

  16. Ezura H, Kikuta I, Oosawa K (1994) Production of aneuploid melon plants following in vitro culture of seeds from a triploid x diploid cross. Plant Cell Tissue Organ Cult 38:61–63

    Article  Google Scholar 

  17. Beharav A, Cohen Y (1995) Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L. Plant Growth Regul 16:267–269

    Article  CAS  Google Scholar 

  18. Metwally EI, Moustafa SA, El-Sawy BI, Shalaby TA (1998) Haploid plantlets derived by anther culture of Cucurbita pepo. Plant Cell Tissue Organ Cult 52:171–176

    Article  CAS  Google Scholar 

  19. Sauton A (1988) Effect of season and genotype on gynogenetic haploid production in muskmelon, Cucumis melo L. Sci Hortic (Amsterdam) 35:71–75

    Article  Google Scholar 

  20. Metwally EI, Moustafa SA, El-Sawy BI, Haroun SA, Shalaby TA (1998) Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo. Plant Cell Tissue Organ Cult 52:117–121

    Article  CAS  Google Scholar 

  21. Gémes-Juhász A, Balogh P, Ferenczy A, Kristóf Z (2002) Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Rep 21:105–111

    Article  Google Scholar 

  22. Norton JD (1981) Embryo culture of Cucumis species. HortScience 16:69

    Google Scholar 

  23. Dryanovska OA, Ilieva IN (1983) In vitro anther and ovule cultures in muskmelon (Cucumis melo L.). C R Acad Bulg Sci 36:1107–1110

    Google Scholar 

  24. Lazarte JE, Sasser CC (1982) Asexual embryogenesis and plantlet development in anther culture of Cucumis sativus L. HortScience 17:88

    Google Scholar 

  25. Przyborowski J, Niemirowicz-Szczytt K (1994) Main factors affecting cucumber (Cucumis sativus L.) haploid embryo development and haploid plant characteristics. Plant Breed 112:70–75

    Article  Google Scholar 

  26. Chen JF, Staub JE, Tashiro Y, Isshiki S, Miyazaki S (1997) Successful interspecific hybridization between Cucumis sativus L. and C. hystrix Chakr. Euphytica 96:413–419

    Article  Google Scholar 

  27. Malepszy S, Sarreb DA, Mackiewicz HO, Narkiewicz M (1998) Triploids in cucumber: I. Factors influencing embryo rescue efficiency. Gartenbauwissenschaft 63:34–37

    Google Scholar 

  28. Chen JF, Staub J, Adelberg J, Lewis S, Kunkle B (2002) Synthesis and preliminary characterization of a new species (amphidiploid) in Cucumis. Euphytica 123:315–322

    Article  Google Scholar 

  29. Ondrej V, Nvrátilová B, Lebeda A (2002) In vitro cultivation of Cucumis sativus ovules after fertilization. Acta Hortic 588:339–343

    CAS  Google Scholar 

  30. Chen J, Staub J, Qian C, Jiang J, Luo X, Zhuang F (2003) Reproduction and cytogenetic characterization of interspecific hybrids derived from Cucumis hystrix Chakr. x Cucumis sativus L. Theor Appl Genet 106:688–695

    PubMed  CAS  Google Scholar 

  31. Ashok-Kumar HG, Murthy HN, Paek KY (2003) Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. Sci Hortic (Amsterdam) 98:213–222

    Article  CAS  Google Scholar 

  32. Gémes-Juhász A, Venczel G, Sági Z, Gajdos L, Zatykó L, Kristóf Z, Vági P (2006) Production of doubled haploid breeding lines in case of paprika, spice paprika, eggplant, cucumber, zucchini and onion. Acta Hortic 725:845–853

    Google Scholar 

  33. Dolcet-Sanjuan R, Claveria E, Garcia-Mas J (2006) Cucumber (Cucumis sativus L.) dihaploid line production using in vitro ­rescue of in vivo induced parthenogenic embryos. Acta Hortic 725:837–844

    CAS  Google Scholar 

  34. Nuñez-Palenius HG, Klee HJ, Cantliffe D (2006) Embryo rescue culture of the ‘Galia’ muskmelon (Cucumis melo L. var. reticulatus Ser.) male parental line. Plant Cell Tissue Organ Cult 85:345–352

    Article  Google Scholar 

  35. Skálová D, Dziechciarková M, Lebeda A, Navrátilová B, Krístková E (2007) Interspecific hybridization of C. anguria x C. zeyheri, C. sativus x C. melo, and C. sativus x C. metuliferus with the use of embryo cultures. Acta Hortic 731:77–82

    Google Scholar 

  36. Skálová D, Dziechciarková M, Lebeda A, Krístková E, Navrátilová B (2008) Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue. Biol Plant 52:775–778

    Article  Google Scholar 

  37. Smiech M, Sztangret-Wisniewska J, Galecka T, Korzeniewska A, Marzec L, Kolakowska G, Piskurewicz U, Niemirowicz-Szczytt K (2008) Potential use of RAPD markers in characteristics of cucumber (Cucumis sativus L.) haploids and double-haploids. Acta Soc Bot Pol 77:29–34

    CAS  Google Scholar 

  38. Skálová D, Navratilova B, Lebeda A (2008) Embryo rescue of cucumber (Cucumis sativus), muskmelon (C. melo) and some wild Cucumis species (C. anguria, C. zeyheri, and C. metuliferus). J Appl Bot Food Qual 82:83–89

    Google Scholar 

  39. Mackiewicz HO, Malepszy S, Sarreb DA, Narkiewicz M (1998) Triploids in cucumber: II. Characterization of embryo rescue plants. Gartenbauwissenschaft 63:125–129

    Google Scholar 

  40. Kumar HGA, Murthy HN, Paek KY (2003) Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. Sci Hortic (Amsterdam) 98:213–222

    Article  Google Scholar 

  41. Ondrej V, Navratilova B, Lebeda A (2002) Influence of GA3 on the zygotic embryogenesis of Cucumis species in vitro. Biologia 57:523–525

    CAS  Google Scholar 

  42. Ezura H, Amagai H, Oosawa K (1993) Efficient production of triploid melon plants by in-vitro culture of abnormal embryos excised from dried seeds of diploid x tetraploid crosses and their characteristics. Jpn J Breed 43:193–199

    Google Scholar 

  43. Cuny F, Grotte M, Devaulx RD, Rieu A (1993) Effects of gamma-irradiation of pollen on parthenogenetic haploid production in muskmelon (Cucumis melo L.). Environ Exp Bot 33:301–312

    Article  Google Scholar 

  44. Diao WP, Jia YY, Song H, Zhang XQ, Lou QF, Chen JF (2009) Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenerants using SSR markers. Sci Hortic (Amsterdam) 119:246–251

    Article  CAS  Google Scholar 

  45. Robinson RW, Decker-Walters DS (1999) Cucurbits. CAB International, Wallingford, NY

    Google Scholar 

  46. Kuzuya M, Hosoya K, Hayato-Masuya Y, Ezura H (2000) Histological observations of powdery mildew resistance in diploid and haploid melons. Acta Hortic 510:71–75

    Google Scholar 

  47. Kuzuya M, Hosoya K, Tomita K, Ezura H (2002) Selection of powdery mildew resistance among haploid plants generated from hybrids of resistant and susceptible melon genotypes. Acta Hortic 588:331–338

    Google Scholar 

  48. Kuzuya M, Hosoya K, Yashiro K, Tomita K, Ezura H (2003) Powdery mildew (Sphaerotheca fuliginia) resistance in melon is selectable at the haploid level. J Exp Bot 54:1069–1074

    Article  PubMed  CAS  Google Scholar 

  49. Ficcadenti N, Sestili S, Annibali S, Campanelli G, Belisario A, Maccaroni M, Corazza L (2002) Resistance to Fusarium oxysporum f. sp. melonis race 1, 2 in muskmelon lines Nad-1 and Nad-2. Plant Dis 86:897–900

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav)-Unidad Irapuato, Irapuato, Gto, Mexico

    Hector Gordon Nuñez-Palenius & Neftalí Ochoa-Alejo

  2. Departamento de Agronomía. División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Irapuato, Gto, México

    Rafael Ramírez-Malagón

Authors
  1. Hector Gordon Nuñez-Palenius
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rafael Ramírez-Malagón
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Neftalí Ochoa-Alejo
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Dept. Biological Sciences, University of Calgary, University Dr. NW., 2500, Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

  2. , Department of Biological Sciences, University of Calgary, University Dr. NW 2500, Calgary, T2N 1N4, Alberta, Canada

    Edward C. Yeung

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Humana Press

About this protocol

Cite this protocol

Nuñez-Palenius, H.G., Ramírez-Malagón, R., Ochoa-Alejo, N. (2011). Muskmelon Embryo Rescue Techniques Using In Vitro Embryo Culture. In: Thorpe, T., Yeung, E. (eds) Plant Embryo Culture. Methods in Molecular Biology, vol 710. Humana Press. https://doi.org/10.1007/978-1-61737-988-8_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-61737-988-8_9

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-987-1

  • Online ISBN: 978-1-61737-988-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation