Multiparametric Analysis of Apoptosis by Flow Cytometry
- William G. TelfordAffiliated withFACS Core Facilty, Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health
- , Akira KomoriyaAffiliated withOncoImmunin, Inc.
- , Beverly Z. PackardAffiliated withOncoImmunin, Inc.
- , C. Bruce BagwellAffiliated withVerity Software House
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Flow cytometry is the most widely used technology for analyzing apoptosis. The multiparametric nature of flow cytometry allows several apoptotic characteristics to be combined in a single sample, making it a powerful tool for analyzing the complex progression of apoptotic death. This chapter provides guidelines for combining caspase detection, annexin V binding, DNA dye exclusion, and other single apoptotic assays into multiparametric assays.
This approach to analyzing apoptosis provides far more information than single parameter assays that provide only an ambiguous “percent apoptotic” result, given that multiple early, intermediate and late apoptotic stages can be visualized simultaneously. This multiparametric approach is also amenable to a variety of flow cytometric instrumentation, both old and new.
Key wordsApoptosis Caspase Flow cytometry Annexin V 7-Aminoactinomycin D Propidium iodide Pacific Blue Hoechst 33258
- Multiparametric Analysis of Apoptosis by Flow Cytometry
- Book Title
- Flow Cytometry Protocols
- pp 203-227
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series Subtitle
- Methods and Protocols
- Series ISSN
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- Additional Links
- Flow cytometry
- Annexin V
- 7-Aminoactinomycin D
- Propidium iodide
- Pacific Blue
- Hoechst 33258
- Industry Sectors
- Editor Affiliations
- ID1. Medical Center, Flow Cytometry Core Facility, George Washington University
- ID2. Medical Center, Dept. Anatomy & Regenerative Biology, George Washington University
- Author Affiliations
- 1. FACS Core Facilty, Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
- 2. OncoImmunin, Inc., Gaithersburg, MD, USA
- 3. Verity Software House, Topsham, ME, USA
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