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Engineering Protein Stability

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Book cover Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 681))

Abstract

This article defines protein stability, emphasizes its importance and surveys some notable recent publications (2004–2008) in the field of protein stability/stabilization. Knowledge of the factors stabilizing proteins has emerged from denaturation studies and from study of thermophilic (and other extremophilic) proteins. One can enhance stability by protein engineering strategies, the judicious use of solutes and additives, immobilization, and chemical modification in solution. General protocols are set out on how to measure the kinetic thermal stability of a given protein and how to undertake chemical modification of a protein in solution.

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Acknowledgement

The author thanks Dr Barry J. Ryan for helpful and informative discussions.

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Authors and Affiliations

  1. School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland

    Ciarán Ó’Fágáin

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  1. Ciarán Ó’Fágáin
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Correspondence to Ciarán Ó’Fágáin .

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Editors and Affiliations

  1. National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin, 9, Ireland

    Dermot Walls

  2. National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin, 9, Ireland

    Sinéad T. Loughran

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Ó’Fágáin, C. (2011). Engineering Protein Stability. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 681. Humana Press. https://doi.org/10.1007/978-1-60761-913-0_7

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  • DOI: https://doi.org/10.1007/978-1-60761-913-0_7

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