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Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins

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Book cover Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 681))

Abstract

Where an affinity tag has served its purpose it may become desirable to remove it from the protein of interest. This chapter describes the removal of such fusion partners from the intended protein product by cleavage with site-specific endoproteases. Methods to achieve proteolytic cleavage of the fusion proteins are provided, along with techniques for optimising the yield of authentic product.

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Author information

Authors and Affiliations

  1. Commonwealth Serum Laboratories Ltd, ParkvilleVIC, Australia

    Adam Charlton

  2. BioMarin Pharmaceutical Inc, Novato, CA, USA

    Michael Zachariou

Authors
  1. Adam Charlton
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  2. Michael Zachariou
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Editor information

Editors and Affiliations

  1. National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin, 9, Ireland

    Dermot Walls

  2. National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin, 9, Ireland

    Sinéad T. Loughran

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Charlton, A., Zachariou, M. (2011). Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 681. Humana Press. https://doi.org/10.1007/978-1-60761-913-0_19

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  • DOI: https://doi.org/10.1007/978-1-60761-913-0_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-912-3

  • Online ISBN: 978-1-60761-913-0

  • eBook Packages: Springer Protocols

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