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Vaccine Adjuvants pp 59–72Cite as

Freund’s Complete and Incomplete Adjuvants, Preparation, and Quality Control Standards for Experimental Laboratory Animals Use

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 626))

Abstract

Quality control and quality assurance procedures are discussed for the agreed benchmark standard Freund’s complete adjuvant (FCA). In addition, the use of the incomplete adjuvant (FIA) in the preparation of antisera is discussed. A major problem is the use of a safe and suitable mineral oil in FCA and FIA; manufacturers should provide infra-red spectra and gas liquid chromatography analyses. A range of safety tests, toxicity, pyrogenicity and endotoxin assays and advice on practical procedures for the use of these adjuvants are described.

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Authors and Affiliations

  1. Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, Scotland

    Duncan E.S. Stewart-Tull

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  1. Duncan E.S. Stewart-Tull
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Correspondence to Duncan E.S. Stewart-Tull .

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Editors and Affiliations

  1. Dept. Cardio & Vascular Sciences, St. George's University of London, Cranmer Terrace, London, SW17 0RE, United Kingdom

    Gwyn Davies

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Stewart-Tull, D.E. (2010). Freund’s Complete and Incomplete Adjuvants, Preparation, and Quality Control Standards for Experimental Laboratory Animals Use. In: Davies, G. (eds) Vaccine Adjuvants. Methods in Molecular Biology, vol 626. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-585-9_5

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  • DOI: https://doi.org/10.1007/978-1-60761-585-9_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-584-2

  • Online ISBN: 978-1-60761-585-9

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