Abstract
Antibody titre is a measure of the presence and amount of antibodies specific to an antigen that are present in the blood. In particular, the titre of an antibody sample is a measure of the antibody concentration determined under a defined set of conditions, with the antibody concentration being commonly established by enzyme-linked immunosorbent assay, also called ELISA, or a variation of this technology.Enzyme-linked immunosorbent assay, also called ELISA, enzyme immunoassay or EIA, is a biochemical technique used to detect the presence of an antibody or an antigen in a sample. The ELISA/EIA approach is an extremely robust technology and readily amenable to validation and quality control if adherence to ISO quality standards is required. This chapter describes in detail a specific ELISA protocol which has potential generic application.
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The help of Paul Risley and Patrick Corran finalising the details of this document is gratefully acknowledged.
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Walker, B., Feavers, I. (2010). Adjuvant Effects on Antibody Titre. In: Davies, G. (eds) Vaccine Adjuvants. Methods in Molecular Biology, vol 626. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-585-9_13
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DOI: https://doi.org/10.1007/978-1-60761-585-9_13
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Publisher Name: Humana Press, Totowa, NJ
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