Abstract
The development of techniques and methods for allergen purification is essential for diagnosis and the development of safe immunotherapeutic agents. The most common purification techniques include chromatographic methodologies. In this chapter, we review and describe the details of the methodologies of using ion-exchange, gel-filtration, and affinity chromatography to purify two well-known panallergens, profilin and parvalbumin.
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Acknowledgement
This work was supported by grants from the Instituto de Salud Carlos III (PI 13/00477, PI 13/00928), co-supported by FONDOS FEDER and the Institute of Applied Molecular Medicine of CEU San Pablo University of Madrid.
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Cases, B., Pastor-Vargas, C., Perez-Gordo, M. (2017). Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques. In: Lin, J., Alcocer, M. (eds) Food Allergens. Methods in Molecular Biology, vol 1592. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6925-8_2
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DOI: https://doi.org/10.1007/978-1-4939-6925-8_2
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