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Biosensors and Biodetection pp 73–88Cite as

Biosensing Based on Magneto-Optical Surface Plasmon Resonance

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1571))

Abstract

In spite of the high analytic potential of Magneto Optical Surface Plasmon Resonance (MOSPR) assays, their applicability to biosensing has been limited due to significant chip stability issues. We present novel solutions to surpass current limitations of MOSPR sensing assays, based on innovative chip structure, tailored measurements and improved data analysis methods. The structure of the chip is modified to contain a thin layer of Co-Au alloy instead of successive layers of homogenous metals with magnetic and plasmonic properties, as currently used. This new approach presents improved plasmonic and magnetic properties, yet a structural stability similar to standard Au-SPR chips, allowing for bioaffinity assays in saline solutions. Moreover, using a custom-designed measurement configuration that allows the acquisition of the SPR curve, i.e., the reflectivity measured at multiple angles of incidence, instead of the reflectivity value at a single-incidence angle, a high signal-to-noise ratio is achieved, suitable for detection of minute analyte concentrations. The proposed structure of the MOSPR sensing chip and the procedure of data analysis allow for long time assessment in liquid media, a significant advancement over existing MOSPR chips, and confirm the MOSPR increased sensitivity over standard SPR analyses.

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Acknowledgments

The authors thank the Romanian Executive Unit for Higher Education, Research, Development and Innovation Funding for funding through grants PN II-ID-PCCE-2011-2-0075 and PN-II-RU-PD-2012-3-0467.

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Authors and Affiliations

  1. International Centre of Biodynamics, Intrarea Portocalelor 1B, Bucharest, 060101, Romania

    Sorin David, Cristina Polonschii, Mihaela Gheorghiu, Dumitru Bratu & Eugen Gheorghiu

  2. University of Bucharest, 4-12 Regina Elisabeta Blvd, Bucharest, 030018, Romania

    Eugen Gheorghiu

Authors
  1. Sorin David
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  2. Cristina Polonschii
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  3. Mihaela Gheorghiu
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  4. Dumitru Bratu
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  5. Eugen Gheorghiu
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Corresponding author

Correspondence to Eugen Gheorghiu .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

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David, S., Polonschii, C., Gheorghiu, M., Bratu, D., Gheorghiu, E. (2017). Biosensing Based on Magneto-Optical Surface Plasmon Resonance. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6848-0_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6846-6

  • Online ISBN: 978-1-4939-6848-0

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