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Biosensors and Biodetection

Volume 1571 of the series Methods in Molecular Biology pp 357-369

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A Novel Colorimetric PCR-Based Biosensor for Detection and Quantification of Hepatitis B Virus

  • Li YangAffiliated withNatural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science
  • , Mei LiAffiliated withNatural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science
  • , Feng DuAffiliated withNatural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science
  • , Gangyi ChenAffiliated withNatural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science
  • , Afshan  YasmeenAffiliated withNatural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science
  • , Zhuo TangAffiliated withNatural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science Email author 

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Abstract

Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that puts people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has infected one-third of the worldwide population, and 350 million people suffer from chronic HBV infection. For these reasons, development of an accurate, sensitive, and expedient detection method for diagnosing, monitoring, and assessing therapeutic response of HBV is very necessary and urgent for public health and disease control. Here we report a new strategy for detection of viral load quantitation of HBV based on colorimetric polymerase chain reaction (PCR) with DNAzyme-containing probe. The special DNAzyme adopting a G-quadruplex structure exhibited peroxidase-like activity in the presence of hemin to report colorimetric signal. This method has shown a broad range of linearity and high sensitivity. This study builds an important foundation to achieve the specific and accurate detection level of HBV DNA with a low-cost and effective method in helping diagnosing, preventing and protecting human health form HBV all over the world, and especially in developing countries.

Key words

Hepatitis B virus Molecular diagnosis Biosensor G-quadruplex DNAzyme Peroxidase-like activity Colorimetric PCR