Abstract
Quantitative assessment of adipose mitochondrial activity is critical for better understanding of adipose tissue function in obesity and diabetes. While the two-dimensional (2-D) tissue culture method has been sufficient to discover key molecules that regulate adipocyte differentiation and function, the method is insufficient to determine the role of extracellular matrix (ECM) molecules and their modifiers, such as matrix metalloproteinases (MMPs), in regulating adipocyte function in three-dimensional (3-D) in vivo-like microenvironments. By using a 3-D hanging drop tissue culture system, we are able to produce scalable 3-D adipospheres that are suitable for quantitative metabolic study in 3-D microenvironment.
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Acknowledgment
S.T. has stock options in 3D Biomatrix, a company commercializing the hanging drop plate technology. This research was funded by NIH R01DK095137 (T.H.C.).
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Akama, T., Leung, B.M., Labuz, J., Takayama, S., Chun, TH. (2017). Designing 3-D Adipospheres for Quantitative Metabolic Study. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_17
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DOI: https://doi.org/10.1007/978-1-4939-6820-6_17
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Publisher Name: Humana Press, New York, NY
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