Abstract
Inter-organ communication is central to mammalian metabolism, allowing for the coordination of crucial homeostatic processes such as food intake, body weight, and blood glucose. Dissecting this process at a cellular and molecular level requires the establishment of a reductionist system in which cell types of interest can be investigated as the source and target of biologically relevant signals. Here, we describe a system to study the interaction between adipocytes and hepatocytes using either conditioned media from cultured adipocytes or co-culture of hepatocytes with adipose explants. These methods have the potential to identify novel polypeptides and metabolites involved in the adipose-liver metabolic axis.
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O’Connor, S., Cohen, P. (2017). In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_15
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DOI: https://doi.org/10.1007/978-1-4939-6820-6_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6819-0
Online ISBN: 978-1-4939-6820-6
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