Abstract
The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Che H, Yue J, Tse HF, Li GR (2014) Functional TRPV and TRPM channels in human preadipocytes. Pflugers Arch 466(5):947–959
Sukumar P et al (2012) Constitutively active TRPC channels of adipocytes confer a mechanism for sensing dietary fatty acids and regulating adiponectin. Circ Res 111(2):191–200
Ye L et al (2012) TRPV4 is a regulator of adipose oxidative metabolism, inflammation, and energy homeostasis. Cell 151(1):96–110
Gardner P (1990) Patch clamp studies of lymphocyte activation. Annu Rev Immunol 8:231–250
Molleman A (2003) Patch clamping: an introductory guide to patch clamp electrophysiology. Wiley, New York
Sakmann B, Neher E (1984) Patch clamp techniques for studying ionic channels in excitable membranes. Annu Rev Physiol 46:455–472
Hille B (2001) Ion channels of excitable membranes, 3rd edn. Sinauer Associates, Sunderland, MA
Acknowledgment
This work was supported by a grant from the American Heart Association and from the Roy Carver Charitable Trust to R.S.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Zhang, Y. et al. (2017). Isolation and Patch-Clamp of Primary Adipocytes. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6820-6_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6819-0
Online ISBN: 978-1-4939-6820-6
eBook Packages: Springer Protocols