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Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry

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Thermogenic Fat

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1566))

Abstract

Global obesity epidemic demands more effective therapeutic treatments and better understanding of obesity pathophysiology. Since obesity results from energy imbalance, accurate quantification of energy intake and energy expenditure (EE) becomes an essential prerequisite to phenotype the cause for obesity development. Indirect calorimetry has long been used as one of the most established methods in EE quantification by detecting changes in levels of O2 consumption and CO2 production. In this article, we describe procedures and important considerations for an effective measurement using indirect calorimetry.

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Acknowledgment

This work was supported by NIH R01DK092605. Q.T. is the holder of Cullen Chair in Molecular Medicine and Welch Research Scholar (L-AU0002) of the University of Texas McGovern Medical School.

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Authors and Affiliations

  1. Brown Foundation Institute of Molecular Medicine, University of Texas McGovern Medical School, 1825 Pressler St., SRB 430G, Houston, TX, 77030, USA

    Eun Ran Kim & Qingchun Tong

  2. Department of Neurobiology and Anatomy, University of Texas McGovern Medical School, Houston, TX, 77030, USA

    Qingchun Tong

Authors
  1. Eun Ran Kim
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  2. Qingchun Tong
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Corresponding author

Correspondence to Qingchun Tong .

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Editors and Affiliations

  1. Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA

    Jun Wu

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Kim, E.R., Tong, Q. (2017). Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6820-6_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6819-0

  • Online ISBN: 978-1-4939-6820-6

  • eBook Packages: Springer Protocols

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