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Thermogenic Fat

Volume 1566 of the series Methods in Molecular Biology pp 109-124

Date:

Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

  • Nicolás Gómez-BanoyAffiliated withMetabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine
  • , James C. LoAffiliated withMetabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine Email author 

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Abstract

Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.

Key words

Adipose tissue Brown fat White fat Beige fat Adipocyte Viral vectors Adenovirus Transduction Retrovirus Lentivirus Adeno-associated virus AAV Adipogenesis Adipokine Metabolism Diabetes Obesity