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Histochemistry of Single Molecules

Volume 1560 of the series Methods in Molecular Biology pp 133-144

Date:

The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

  • Vicente Seco-RoviraAffiliated withDepartment of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia
  • , Ester Beltrán-FrutosAffiliated withDepartment of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia
  • , Jesús Martínez-HernándezAffiliated withDepartment of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia
  • , Concepción FerrerAffiliated withDepartment of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia
  • , Luis Miguel PastorAffiliated withDepartment of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia Email author 

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Abstract

Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.

Key words

Lectin histochemistry TUNEL Apoptosis Testis Germ cell Syrian hamster Sensitivity