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Enzyme Histochemistry for Functional Histology in Invertebrates

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Book cover Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1560))

Abstract

In invertebrates, enzyme histochemistry has recently found a renaissance regarding its applications in morphology and ecology. Many enzyme activities are useful for the morphofunctional characterization of cells, as biomarkers of biological and pathologic processes, and as markers of the response to environmental stressors. Here, the adjustments to classic techniques, including the most common enzymes used for digestion, absorption, transport, and oxidation, as well as techniques for azo-coupling, metal salt substitution and oxidative coupling polymerization, are presented in detail for various terrestrial and aquatic invertebrates. This chapter also provides strategies to solve the problems regarding anesthesia, small body size, the presence of an exo- or endoskeleton and the search for the best fixative in relation to the internal fluid osmolarity. These techniques have the aim of obtaining good results for both the pre- and post-embedding labeling of specimens, tissue blocks, sections, and hemolymph smears using both light and transmission electron microscopy.

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Authors and Affiliations

  1. Department of Biology, University of Padua, Via Ugo Bassi 58/B, 35131, Padova, Italy

    Francesca Cima

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  1. Francesca Cima
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Correspondence to Francesca Cima .

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Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Marco Biggiogera

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Cima, F. (2017). Enzyme Histochemistry for Functional Histology in Invertebrates. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6788-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6787-2

  • Online ISBN: 978-1-4939-6788-9

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