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Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications

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Book cover Signal Transduction Immunohistochemistry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1554))

Abstract

Phosphate addition is a posttranslational modification of proteins, and this modification can affect the activity and other properties of intracellular proteins. Different animal species can be used to generate phosphosite-specific antibodies as either polyclonals or monoclonals, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is vital for their use in proteomics and profiling of disease.

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  1. Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA

    Kathy Brumbaugh, Wen-Chie Liao, J. P. Houchins, Jeff Cooper & Steve Stoesz

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  1. Kathy Brumbaugh
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  2. Wen-Chie Liao
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  3. J. P. Houchins
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Correspondence to Kathy Brumbaugh .

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  1. Immunocytochemistry & Elispot Assays, Bio-Techne, Inc., Minneapolis, Minnesota, USA

    Alexander E. Kalyuzhny

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Brumbaugh, K., Liao, WC., Houchins, J.P., Cooper, J., Stoesz, S. (2017). Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications. In: Kalyuzhny, A. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 1554. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6759-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6759-9_1

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