Abstract
The protocol describes a computational method to develop a Single Locus Sequence Typing (SLST) scheme for typing bacterial species. The resulting scheme can be used to type bacterial isolates as well as bacterial species directly from complex communities using next-generation sequencing technologies.
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References
Gruber N, Galloway JN (2008) An Earth-system perspective of the global nitrogen cycle. Nature 451:293–296
Cho I, Blaser MJ (2012) The human microbiome: at the interface of health and disease. Nat Rev Genet 13:260–270
Hartstra AV, Bouter KEC, Bäckhed F, Nieuwdorp M (2015) Insights into the role of the microbiome in obesity and type 2 diabetes. Diabetes Care 38:159–165
Ley RE (2010) Obesity and the human microbiome. Curr Opin Gastroenterol 26:5–11
Proal AD, Albert PJ, Marshall TG (2013) The human microbiome and autoimmunity. Curr Opin Rheumatol 25:234–240
Round JL, Mazmanian SK (2009) The gut microbiota shapes intestinal immune responses during health and disease. Nat Rev Immunol 9:313–323
Liu B, Faller LL, Klitgord N et al (2012) Deep sequencing of the oral microbiome reveals signatures of periodontal disease. PLoS ONE 7:e37919
Peterson SN, Snesrud E, Liu J et al (2013) The dental plaque microbiome in health and disease. PLoS ONE 8:e58487
Wade WG (2013) The oral microbiome in health and disease. Pharmacol Res 69:137–143
Haubek D, Ennibi O-K, Poulsen K et al (2008) Risk of aggressive periodontitis in adolescent carriers of the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans in Morocco: a prospective longitudinal cohort study. Lancet 371:237–242
Kazakova SV, Hageman JC, Matava M et al (2005) A clone of methicillin-resistant Staphylococcus aureus among professional football players. N Engl J Med 352:468–475
Karch H, Tarr PI, Bielaszewska M (2005) Enterohaemorrhagic Escherichia coli in human medicine. Int J Med Microbiol 295:405–418
Sabat AJ, Budimir A, Nashev D et al (2013) Overview of molecular typing methods for outbreak detection and epidemiological surveillance. Euro Surveill 18:20380
Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 106:19126–19131
Scholz CFP, Poulsen K, Kilian M (2012) Novel molecular method for identification of Streptococcus pneumoniae applicable to clinical microbiology and 16S rRNA sequence-based microbiome studies. J Clin Microbiol 50:1968–1973
Maiden MCJ (2006) Multilocus sequence typing of bacteria. Annu Rev Microbiol 60:561–588
Maiden MCJ, Bygraves JA, Feil E et al (1998) Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 95:3140–3145
Bishop CJ, Aanensen DM, Jordan GE et al (2009) Assigning strains to bacterial species via the internet. BMC Biol 7:3
Gevers D, Cohan FM, Lawrence JG et al (2005) Re-evaluating prokaryotic species. Nat Rev Microbiol 3:733–739
Glaeser SP, Kämpfer P (2015) Multilocus sequence analysis (MLSA) in prokaryotic taxonomy. Syst Appl Microbiol 38:237–245
Sawabe T, Kita-Tsukamoto K, Thompson FL (2007) Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189:7932–7936
Shendure J, Ji H (2008) Next-generation DNA sequencing. Nat Biotechnol 26:1135–1145
Wang Y, Qian P-Y (2009) Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. PLoS ONE 4:e7401
Schloss PD, Westcott SL (2011) Assessing and improving methods used in operational taxonomic unit-based approaches for 16S rRNA gene sequence analysis. Appl Environ Microbiol 77:3219–3226
Eren AM, Borisy GG, Huse SM, Mark Welch JL (2014) Oligotyping analysis of the human oral microbiome. Proc Natl Acad Sci U S A 111:E2875–E2884
Chen T, Yu W-H, Izard J et al (2010) The human oral microbiome database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database 2010:baq013.
Conlan S, Kong HH, Segre JA (2012) Species-level analysis of DNA sequence data from the NIH human microbiome project. PLoS ONE 7:e47075
Jensen A, Fagö-Olsen H, Sørensen CH, Kilian M (2013) Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis. PLoS ONE 8:e56418
Tu Q, He Z, Zhou J (2014) Strain/species identification in metagenomes using genome-specific markers. Nucleic Acids Res 42:e67
Joseph SJ, Li B, Petit RA et al (2015) The single-species metagenome: subtyping Staphylococcus aureus core genome sequences from shotgun metagenomic data. bioRxiv 030692
Drevinek P, Vosahlikova S, Dedeckova K et al (2010) Direct culture-independent strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis. J Clin Microbiol 48:1888–1891
Scholz CFP, Jensen A, Lomholt HB et al (2014) A novel high-resolution single locus sequence typing scheme for mixed populations of Propionibacterium acnes in vivo. PLoS ONE 9:e104199
Lomholt HB, Kilian M (2010) Population genetic analysis of Propionibacterium acnes identifies a subpopulation and epidemic clones associated with acne. PLoS ONE 5:e12277
Welcome to Python.org. In: Python.org. https://www.python.org/. Accessed 28 Nov 2015
Camacho C, Coulouris G, Avagyan V et al (2009) BLAST+: architecture and applications. BMC Bioinformatics 10:421
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797
Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Okonechnikov K, Golosova O, Fursov M, UGENE Team (2012) Unipro UGENE: a unified bioinformatics toolkit. Bioinformatics 28:1166–1167
Ye J, Coulouris G, Zaretskaya I et al (2012) Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics 13:134
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Scholz, C.F.P., Jensen, A. (2017). Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities. In: Nordenfelt, P., Collin, M. (eds) Bacterial Pathogenesis. Methods in Molecular Biology, vol 1535. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6673-8_7
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DOI: https://doi.org/10.1007/978-1-4939-6673-8_7
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