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Bacterial Pathogenesis

Volume 1535 of the series Methods in Molecular Biology pp 197-209

Date:

Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy

  • Amy L. BottomleyAffiliated withThe ithree institute, University of Technology Sydney 
  • , Lynne TurnbullAffiliated withThe ithree institute, University of Technology Sydney 
  • , Cynthia B. WhitchurchAffiliated withThe ithree institute, University of Technology Sydney
  • , Elizabeth J. HarryAffiliated withThe ithree institute, University of Technology Sydney Email author 

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Abstract

Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.

Key words

Bacterial slide preparation Agarose pads GelGro slabs Super-resolution microscopy Fast three-dimensional structured illumination microscopy