Abstract
Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.
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Acknowledgments
We thank Servier Medical Art (http://www.servier.com/Powerpoint-image-bank) for providing drawings used in Fig. 1. Work in the laboratory of P.C. is supported by the Institut Pasteur (Unité des Interactions Bactéries-Cellules), the Institut National de la Santé et de la Recherche Médicale (Unité 604), the Institut National de la Recherche Agronomique (Unité Sous Contrat 2020), the Fondation Les Mousquetaires, and an European Research Council Advanced Grant (BacEpiCell 670823). The Imagopole is part of the FranceBioImaging infrastructure supported by the French National Research Agency (ANR-10-INSB-04-01, “Investments for the Future”) and is grateful to supports by Conseil de la Region Ile-de-France (program Sesame 2007, project Imagopole to S.S.) and from the Fondation Française pour la Recherche Médicale (FRM, Programme Grands Equipements to N.A.). J.E. is supported by the Institut Pasteur (PTR-460), by the Institut Pasteur Carnot-MIE program and by a European Research Council Starting Grant (RuptEffects 261166). J.P.C. is supported by the Institut Pasteur (PTR-460 and PTR-521) and by the French National Research Agency (ANR-15-CE15-0017). P.C. and J.E. acknowledge the LabEx consortium IBEID. P.C. is a Howard Hughes Medical Institute Senior International Research Scholar.
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Quereda, J.J. et al. (2017). Assessing Vacuolar Escape of Listeria Monocytogenes . In: Nordenfelt, P., Collin, M. (eds) Bacterial Pathogenesis. Methods in Molecular Biology, vol 1535. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6673-8_11
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DOI: https://doi.org/10.1007/978-1-4939-6673-8_11
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