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Oncogene-Induced Senescence pp 53–68Cite as

Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1534))

Abstract

MicroRNAs are small noncoding RNAs that regulate gene expression by binding to sequences within the 3′-UTR of mRNAs. Genome-wide screens have proven powerful in associating genes with certain phenotypes or signal transduction pathways and thus are valuable tools to define gene function. Here we describe a genome-wide miRNA screening strategy to identify miRNAs that are required to bypass oncogene-induced senescence.

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Acknowledgments

This work was supported by grants from the Spanish Ministry of Economy and Competitiveness, Plan Nacional de I + D + I 2008-2011, Plan Estatal de I + D + I 2013–2016, ISCIII (Fis: PI12/00137, PI15/00045, RTICC: RD12/0036/0028) co-funded by FEDER from Regional Development European Funds (European Union), Consejeria de Ciencia e Innovacion (CTS-6844 and CTS-1848), and Consejeria de Salud of the Junta de Andalucia (PI-0135-2010, PI-0306-2012 and PI-0096-2014).

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Authors and Affiliations

  1. Musculoskeletal and Oncology Lab, Department of Orthopaedics and Rehabilitation, University of Florida, 1600 Archer Road, MSB M2-212, Gainesville, FL, 32610, USA

    Maria V. Guijarro

  2. Molecular Biology of Cancer Group, Oncohematology and Genetic Department, Instituto de Biomedicina de Sevilla (IBIS/HUVR/CSIC/Universidad de Sevilla), Campus HUVR, Edificio IBIS, Avda. Manuel Siurot s/n. 41013, Sevilla, Spain

    Amancio Carnero

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  1. Maria V. Guijarro
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  2. Amancio Carnero
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Correspondence to Maria V. Guijarro or Amancio Carnero .

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Editors and Affiliations

  1. Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York, USA

    Mikhail A. Nikiforov

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Guijarro, M.V., Carnero, A. (2017). Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence. In: Nikiforov, M. (eds) Oncogene-Induced Senescence. Methods in Molecular Biology, vol 1534. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6670-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6670-7_5

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