Abstract
Angiotensin-converting enzyme 2 (ACE2) is a component of the renin-angiotensin system (RAS) which plays an important role in the regulation of blood pressure and volume homeostasis. Accumulating evidence shows alterations in ACE2 expression and activity in several hypertensive animal models, as well as in patients with hypertension. In order to assess the role of brain ACE2 in hypertension, a specific ACE2 assay is required. Based on a quenched fluorescent substrate, we describe an easy-to-use method for determining ACE2 activity in brain tissue and cerebrospinal fluid. The method can further be adapted for other tissues, plasma, cell extracts, and cell culture supernatants.
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Acknowledgments
This work was supported by research grants from the American Heart Association (Established Investigator Award: 12EIA8030004) and National Institutes of Health, National Institute of General Medical Sciences (8 P20 GM103514-10), National Institute of Diabetes and Digestive and Kidney Diseases (DK084466), and National Heart, Lung, and Blood Institute (HL093178) to Dr. Eric Lazartigues.
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Sriramula, S., Pedersen, K.B., Xia, H., Lazartigues, E. (2017). Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_9
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DOI: https://doi.org/10.1007/978-1-4939-6625-7_9
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