Abstract
Functional genomics requires an understanding of the complete network of changes within an organism by extensive measurements of moieties from mRNA, proteins, and metabolites. Metabolomics utilizes analytic chemistry tools to profile the complete spectrum of metabolites found in a tissue, cells, or biofluids using a wide range of tools from infrared spectroscopy, fluorescence spectroscopy, NMR spectroscopy, and mass spectrometry. In this protocol, we outline a procedure for performing metabolomic analysis of urine samples using liquid chromatography–mass spectrometry (LC-MS). We outline the advantages of using this approach and summarize some of the early promising studies in cardiovascular diseases using this approach.
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Tsiropoulou, S., McBride, M., Padmanabhan, S. (2017). Urine Metabolomics in Hypertension Research. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_5
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DOI: https://doi.org/10.1007/978-1-4939-6625-7_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6623-3
Online ISBN: 978-1-4939-6625-7
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