Skip to main content
SpringerLink
Log in
Book cover

Hypertension pp 53–60Cite as

Tissue Proteomics in Vascular Disease

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1527))

Abstract

In vascular diseases, as in many other pathophysiological conditions, tissue proteins are subjected to a number of different changes such as protein expression, posttranslational modifications, and proteolytic cleavage. For this reason, the study of the tissue proteome is becoming an increasingly important tool in biological and clinical research. In this chapter, we describe in detail the methodology for the analysis of tryptic digested peptides from aortic tissue extracts from mice with the aim to elucidate differences in the proteome between control and case or vascular diseased tissue samples. The method encompasses the analysis of these complex extracts in a single chromatographic run allowing for increased reproducibility. We also outline the main workflow for the processing of the generated data by statistical and bioinformatic analysis.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Nordon I, Brar R, Hinchliffe R et al (2009) The role of proteomic research in vascular disease. J Vasc Surg 49:1602–1612

    Article  PubMed  Google Scholar 

  2. Washburn MP, Wolters D, Yates JR (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol 19:242–247

    Article  CAS  PubMed  Google Scholar 

  3. Searle BC (2010) Scaffold: a bioinformatic tool for validating MS/MS-based proteomic studies. Proteomics 10:1265–1269

    Article  CAS  PubMed  Google Scholar 

  4. Thomas S, Bonchev D (2010) A survey of current software for network analysis in molecular biology. Hum Genomics 4:353–360

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Good DM, Thongboonkerd V, Novak J et al (2007) Body fluid proteomics for biomarker discovery: lessons from the past hold the key to success in the future. J Proteome Res 6:549–4555

    Article  Google Scholar 

  6. Lescuyer P, Hochstrasser D, Rabilloud T (2007) How shall we use the proteomics toolbox for biomarker discovery? J Proteome Res 6:3371–3376

    Article  CAS  PubMed  Google Scholar 

  7. Wiśniewski JR, Zougman A, Nagaraj N (2009) Universal sample preparation method for proteome analysis. Nat Methods 6:359–363

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported in part by European grant, HEALTH-2011-278249-EU-MASCARA.

Author information

Authors and Affiliations

  1. School of Natural Sciences, University of Stirling, Stirling, UK

    Amaya Albalat, William Mullen, Holger Husi & Harald Mischak

  2. Mosaiques Diagnostics GmbH, Hannover, Germany

    Harald Mischak

  3. Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK

    Harald Mischak

Authors
  1. Amaya Albalat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William Mullen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Holger Husi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Harald Mischak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Harald Mischak .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  2. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Albalat, A., Mullen, W., Husi, H., Mischak, H. (2017). Tissue Proteomics in Vascular Disease. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6625-7_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6623-3

  • Online ISBN: 978-1-4939-6625-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation