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NADPH Oxidases and Measurement of Reactive Oxygen Species

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Hypertension

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1527))

Abstract

The NADPH oxidase (Nox) family of enzymes is expressed in many tissues that are involved in hypertension, including blood vessels, kidney, and brain. In these tissues, the products of NADPH oxidase activity, superoxide and ultimately hydrogen peroxide, act as intracellular and extracellular messengers during compartmentalized cellular signaling. The correct measurement of Nox activity and its products is crucial to enable studies of how these signaling pathways affect the molecular mechanisms underlying hypertension. Here, we describe methods for detection and measurement of hydrogen peroxide and superoxide derived from NADPH oxidases in biological samples such as cells and tissues.

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Author information

Authors and Affiliations

  1. Division of Cardiology, Department of Medicine, Emory University, 308 WMB, 101 Woodruff Circle, Atlanta, GA, 30322, USA

    Angelica Amanso, Alicia N. Lyle & Kathy K. Griendling Ph.D.

Authors
  1. Angelica Amanso
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  2. Alicia N. Lyle
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  3. Kathy K. Griendling Ph.D.
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Corresponding author

Correspondence to Kathy K. Griendling Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  2. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

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Amanso, A., Lyle, A.N., Griendling, K.K. (2017). NADPH Oxidases and Measurement of Reactive Oxygen Species. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6625-7_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6623-3

  • Online ISBN: 978-1-4939-6625-7

  • eBook Packages: Springer Protocols

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