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Hypertension pp 151–161Cite as

Dopaminergic Immunofluorescence Studies in Kidney Tissue

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1527))

Abstract

The kidney is a highly integrated system of specialized differentiated cells that are responsible for fluid and electrolyte balance in the body. While much of today’s research focuses on isolated nephron segments or cells from nephron segments grown in tissue culture, an often overlooked technique that can provide a unique view of many cell types in the kidney is slice culture. Here, we describe techniques that use freshly excised kidney tissue from rats to perform a variety of experiments shortly after isolating the tissue. By slicing the rat kidney in a “bread loaf” format, multiple studies can be performed on slices from the same tissue in parallel. Cryosectioning and staining of the tissue allow for the evaluation of physiological or biochemical responses in a wide variety of specific nephron segments. The procedures described within this chapter can also be extended to human or mouse kidney tissue.

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Author information

Authors and Affiliations

  1. Department of Pathology, University of Virginia, 22903, Charlottesville, VA, USA

    J. J. Gildea, H. E. McGrath & R. A. Felder

  2. School of Medicine, Fontaine Research Park, University of Virginia, 22903, Charlottesville, VA, USA

    B. A. Kemp & R. M. Carey

  3. Departments of Medicine and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA

    P. A. Jose

  4. Department of Mircobiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia, USA

    R. E. Van Sciver

Authors
  1. J. J. Gildea
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  2. R. E. Van Sciver
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  3. H. E. McGrath
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  4. B. A. Kemp
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  5. P. A. Jose
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  6. R. M. Carey
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  7. R. A. Felder
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Corresponding author

Correspondence to R. A. Felder .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  2. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

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Gildea, J.J. et al. (2017). Dopaminergic Immunofluorescence Studies in Kidney Tissue. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6625-7_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6623-3

  • Online ISBN: 978-1-4939-6625-7

  • eBook Packages: Springer Protocols

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