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Protein and Chromosome Analysis in Mammalian Meiocytes

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Cohesin and Condensin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1515))

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Abstract

Meiosis is a highly specialized cell division that facilitates the production of haploid gametes from diploid mother cells. It is characterized by unique chromatin structures and chromatin associated protein complexes. The analysis of these structures and complexes has greatly benefited from and relied on the visualization of meiotic proteins in diverse preparations of meiocytes. In this chapter we summarize methods that can be used for the characterization of the behavior and localization of meiotic proteins in mammalian meiocytes.

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References

  1. Hodges CA, Hunt PA (2002) Simultaneous analysis of chromosomes and chromosome-associated proteins in mammalian oocytes and embryos. Chromosoma 111(3):165–169

    Article  CAS  PubMed  Google Scholar 

  2. Peters AH et al (1997) A drying-down technique for the spreading of mammalian meiocytes from the male and female germline. Chromosome Res 5(1):66–68

    Article  CAS  PubMed  Google Scholar 

  3. Adelfalk C et al (2009) Cohesin SMC1beta protects telomeres in meiocytes. J Cell Biol 187(2):185–199

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Scherthan H et al (2000) Mammalian meiotic telomeres: protein composition and redistribution in relation to nuclear pores. Mol Biol Cell 11(12):4189–4203

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Wojtasz L et al (2009) Mouse HORMAD1 and HORMAD2, two conserved meiotic chromosomal proteins, are depleted from synapsed chromosome axes with the help of TRIP13 AAA-ATPase. PLoS Genet 5(10):e1000702

    Article  PubMed  PubMed Central  Google Scholar 

  6. Ahmed EA, de Rooij DG (2009) Staging of mouse seminiferous tubule cross-sections. Methods Mol Biol 558:263–277

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fiedlerstraße 42, 01307, Dresden, Germany

    Michelle Stevense, Rolf Jessberger & Attila Tóth

Authors
  1. Michelle Stevense
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  2. Rolf Jessberger
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  3. Attila Tóth
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Corresponding author

Correspondence to Michelle Stevense .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, School of Medicine University of California— Irvine, Irvine, California, USA

    Kyoko Yokomori

  2. Laboratory of Genome Structure and Function Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences The University of Tokyo, Tokyo, Japan

    Katsuhiko Shirahige

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Stevense, M., Jessberger, R., Tóth, A. (2017). Protein and Chromosome Analysis in Mammalian Meiocytes. In: Yokomori, K., Shirahige, K. (eds) Cohesin and Condensin. Methods in Molecular Biology, vol 1515. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6545-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6545-8_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6543-4

  • Online ISBN: 978-1-4939-6545-8

  • eBook Packages: Springer Protocols

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