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Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing

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Cohesin and Condensin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1515))

Abstract

Silenced heterochromatin influences all nuclear processes including chromosome structure, nuclear organization, transcription, replication, and repair. Proteins that mediate silencing affect all of these nuclear processes. Similarly proteins involved in replication, repair, and chromosome structure play a role in the formation and maintenance of silenced heterochromatin. In this chapter we describe a handful of simple tools and methods that can be used to study the atypical role of proteins in gene silencing.

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Authors and Affiliations

  1. Department of Molecular, Cell and Developmental Biology, University of California – Santa Cruz, 249 Sinsheimer Labs, 1156 High St., Santa Cruz, CA, 95064, USA

    Misty R. Peterson, Omar Hamdani & Rohinton T. Kamakaka

  2. School of Biosciences, University of Kent, Canterbury, UK

    Misty R. Peterson

Authors
  1. Misty R. Peterson
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  2. Omar Hamdani
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  3. Rohinton T. Kamakaka
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Correspondence to Rohinton T. Kamakaka .

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Editors and Affiliations

  1. Department of Biological Chemistry, School of Medicine University of California— Irvine, Irvine, California, USA

    Kyoko Yokomori

  2. Laboratory of Genome Structure and Function Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences The University of Tokyo, Tokyo, Japan

    Katsuhiko Shirahige

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Peterson, M.R., Hamdani, O., Kamakaka, R.T. (2017). Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing. In: Yokomori, K., Shirahige, K. (eds) Cohesin and Condensin. Methods in Molecular Biology, vol 1515. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6545-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6545-8_10

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  • Print ISBN: 978-1-4939-6543-4

  • Online ISBN: 978-1-4939-6545-8

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