Abstract
MicroRNAs (miRNAs) are a group of 18–22-nucleotide-long noncoding RNAs, which show wide array of roles in various biological and metabolic processes in both animals and plants. They are formed endogenously and have evolutionary conserved sequences. RNA quality is extremely important for sequencing miRNAs using next-generation sequencing platforms. However, isolation and quantification of miRNAs from plant samples are often technically difficult and recovery of miRNAs from total RNA might be problematic. Degradation of RNA weakens the true miRNAs present in the sample, hence it is crucial to use a protocol that effectively retains good integrity miRNAs. In this chapter, we outline few protocols that can be used to maximize the retrieval of good-quality miRNA and total RNA to be used in miRNA analysis for plants rich in polysaccharides and polyphenols.
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References
MacRae E (2007) Extraction of plant RNA. In: Hilario E, Mackay J (eds) Protocols for nucleic acid analysis by nonradioactive probes, vol 353, 2nd edn, Methods in molecular biology. Humana Press, Totowa, pp 15–24
Wang SX, Hunter W, Plant A (2000) Isolation and purification of functional total RNA from woody branches and needles of Sitka and white spruce. Biotechnique 28:292–296
Geuna F, Hartings H, Scienza A (1998) A new method for rapid extraction of high quality RNA from recalcitrant tissues of grapevine. Plant Mol Biol Rep 16:61–67
Salzman RA, Fugita T, Zhu-Salzman K et al (1999) An improved RNA isolation method for plant tissues containing high levels of phenolic compound or carbohydrates. Plant Mol Biol Rep 17:11–17
Tai HH, Pelletier C, Beardmore T (2004) Total RNA isolation from Pica mariana dry seed. Plant Mol Biol Rep 22:93a–93e
Yeh K, Juang R, Su J (1991) A rapid and efficient method for RNA isolation from plant with high carbohydrate content. Focus 13:102–103
Peng J, Xia Z, Chen L et al (2014) Rapid and efficient isolation of high-quality small RNAs from recalcitrant plant species rich in polyphenols and polysaccharides. PLoS One 9(5), e95687. doi:10.1371/journal.pone.0095687
Yockteng R, Almeida AMR, Yee S, et al (2013) A method for extracting high-quality rna from diverse plants for next generation sequencing and gene expression analysis. Appl Plant Sci 1: Article ID: 13000700
Nadiya F, Anjali N, Gangaprasad A, Sabu KK (2015) High-quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols. Anal Biochem 485:25–27
Cheng H, Gao J, An ZW, Huang HS (2010) A rapid method for isolation of low molecular-weight RNA from Arabidopsis using low salt concentration buffer. Int J Plant Biol 1, e14
Zewei AN, Li Y, XiE L et al (2013) A rapid and economical method for low molecular weight RNA isolation from a wide variety of plant species. Biosci Biotechnol Biochem 77(7):1599–1601
Logemann J, Schell J, Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163:16–20
Asif MH, Dhawan P, Nath P (2000) Plant Mol Biol Rep 18:109–115
Rosas-Cárdenas Fde F, Durán-Figueroa N, Vielle-Calzada JP, Cruz-Hernández A, Marsch-Martínez N, de Folter S (2011) A simple and efficient method for isolating small RNAs from different plant species. Plant Methods 7:4
SchroederA MO, StockerS SR, Leiber M, Gassmann M, Lighfoot S, Menzel W, Granzow M, Ragg T (2006) BMC Mol Biol 7:3
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Sabu, K.K., Nadiya, F., Anjali, N. (2017). MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 1509. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6524-3_4
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DOI: https://doi.org/10.1007/978-1-4939-6524-3_4
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