Abstract
miRNAs are small non-coding RNAs that regulate gene expression and are involved in numerous diseases ranging from osteoporosis, cardiovascular disease, and numerous cancer indications. Most of the clinical material that is accessible for study is archival FFPE blocks. Due to the harsh nature of fixation and embedding procedures involved in preserving clinical material, these samples are heavily fragmented and chemically cross-linked by formalin. miRNAs, due to their small size and increased stability, are easier to retrieve and study in these precious tissues. Therefore, miRNAs have become useful tools in the diagnosis, prognosis, and prediction of diseases. Due to their increased importance, isolation of miRNAs from FFPE material is extremely clinically relevant. Here, we describe the best method for miRNA extraction from FFPE tissue blocks and determine the Qiagen miRNeasy FFPE kit to be the best kit for this task.
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References
Pillai RS (2005) MicroRNA function: multiple mechanisms for a tiny RNA? RNA 11(12):1753–1761. doi:10.1261/rna.2248605
Bentwich I, Avniel A, Karov Y et al (2005) Identification of hundreds of conserved and nonconserved human microRNAs. Nat Genet 37(7):766–770
He L, Thomson JM, Hemann MT et al (2005) A microRNA polycistron as a potential human oncogene. Nature 435(7043):828–833
Lu J, Getz G, Miska EA et al (2005) MicroRNA expression profiles classify human cancers. Nature 435(7043):834–838
Gu S, Kay MA (2010) How do miRNAs mediate translational repression? Silence 1(1):11. doi:10.1186/1758-907X-1-11
Wang Y, Stricker HM, Gou D et al (2007) MicroRNA: past and present. Front Biosci 12:2316–2329, doi:2234 [pii]
Ardekani AM, Naeini MM (2010) The role of MicroRNAs in human diseases. Avicenna J Med Biotechnol 2(4):161–179
Moller HG, Rasmussen AP, Andersen HH et al (2013) A systematic review of microRNA in glioblastoma multiforme: micro-modulators in the mesenchymal mode of migration and invasion. Mol Neurobiol 47(1):131–144. doi:10.1007/s12035-012-8349-7
Ganepola GA, Rutledge JR, Suman P et al (2014) Novel blood-based microRNA biomarker panel for early diagnosis of pancreatic cancer. World J Gastrointest Oncol 6(1):22–33. doi:10.4251/wjgo.v6.i1.22
Søren Jensby Nielsen PD (2010) Screening Tool Can Detect Colorectal Cancer from a Small Blood Sample. American Association for Cancer Research. http://www.aacr.org/home/public--media/aacr-press-releases.aspx?d=2068. Accessed 22 Dec 2010
Liu A, Tetzlaff MT, Vanbelle P et al (2009) MicroRNA expression profiling outperforms mRNA expression profiling in formalin-fixed paraffin-embedded tissues. Int J Clin Exp Pathol 2(6):519–527
Liu A, Xu X (2011) MicroRNA isolation from formalin-fixed paraffin-embedded tissues. Methods Mol Biol 724:259–267. doi:10.1007/978-1-61779-055-3_16
Tanić M, Yanowski K, Andrés E et al (2015) miRNA expression profiling of formalin-fixed paraffin-embedded (FFPE) hereditary breast tumors. Genomics Data 3:75–79. doi:10.1016/j.gdata.2014.11.008
Peng J, Feng Y, Rinaldi G et al (2014) Profiling miRNAs in nasopharyngeal carcinoma FFPE tissue by microarray and Next Generation Sequencing. Genomics Data 2:285–289. doi:10.1016/j.gdata.2014.08.005
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Howe, K. (2017). Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues. In: Rani, S. (eds) MicroRNA Profiling. Methods in Molecular Biology, vol 1509. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6524-3_3
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DOI: https://doi.org/10.1007/978-1-4939-6524-3_3
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