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Covalent Immobilization of Enzymes on Eupergit® Supports: Effect of the Immobilization Protocol

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Enzyme Stabilization and Immobilization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1504))

Abstract

A selection of best combination of adequate immobilization support and efficient immobilization method is still a key requirement for successful application of immobilized enzymes on an industrial level. Eupergit® supports exhibit good mechanical and chemical properties and allow establishment of satisfactory hydrodynamic regime in enzyme reactors. This is advantageous for their wide application in enzyme immobilization after finding the most favorable immobilization method. Methods for enzyme immobilization that have been previously reported as efficient considering the obtained activity of immobilized enzyme are presented: direct binding to polymers via their epoxy groups, binding to polymers via a spacer made from ethylene diamine/glutaraldehyde, and coupling the periodate-oxidized sugar moieties of the enzymes to the polymer beads. The modification of the conventionally immobilized enzyme with ethylenediamine via the carbodiimide route seems to be a powerful tool to improve its stability and catalytic activity.

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Acknowledgments

This work was supported by the Ministry of Science and Technological Development of the Republic of Serbia (Project No III46010).

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Authors and Affiliations

  1. Department for Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia

    Zorica D. Knežević-Jugović, Jelena R. Jovanović, Andrea B. Stefanović, Dejan I. Bezbradica & Dušan Ž. Mijin

  2. Faculty of Technology and Metallurgy, Innovation Centre, University of Belgrade, Karnegijeva 4, Belgrade, Serbia

    Sanja Ž. Grbavčić

  3. Faculty of Technology, University of Novi Sad, Blvd. Cara Lazara 1, Novi Sad, Serbia

    Mirjana G. Antov

Authors
  1. Zorica D. Knežević-Jugović
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  2. Sanja Ž. Grbavčić
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  3. Jelena R. Jovanović
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  4. Andrea B. Stefanović
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  5. Dejan I. Bezbradica
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  6. Dušan Ž. Mijin
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  7. Mirjana G. Antov
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Corresponding author

Correspondence to Zorica D. Knežević-Jugović .

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Editors and Affiliations

  1. Department of Chemistry, and Department of Materials Science and Engineering, University of Utah, Salt Lake City, Utah, USA

    Shelley D. Minteer

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Knežević-Jugović, Z.D. et al. (2017). Covalent Immobilization of Enzymes on Eupergit® Supports: Effect of the Immobilization Protocol. In: Minteer, S. (eds) Enzyme Stabilization and Immobilization. Methods in Molecular Biology, vol 1504. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6499-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6499-4_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6497-0

  • Online ISBN: 978-1-4939-6499-4

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