Abstract
Antibody-dependent cell-mediated cytotoxicity (ADCC) is a relevant characteristic to measure for a number of therapeutic monoclonal antibodies (mAbs) under development. ADCC is a mechanism by which antibody-opsonized, infected, or cancerous cells are destroyed by FcγRIII (CD16)-expressing effector cells. Here we describe three methods that can be used to quantify the ADCC activity of mAbs by measuring distinct aspects of the ADCC mechanism.
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Miller, A.S., Tejada, M.L., Gazzano-Santoro, H. (2014). Methods for Measuring Antibody-Dependent Cell-Mediated Cytotoxicity In Vitro. In: Eggleton, P., Ward, F. (eds) Systemic Lupus Erythematosus. Methods in Molecular Biology, vol 1134. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0326-9_5
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DOI: https://doi.org/10.1007/978-1-4939-0326-9_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0325-2
Online ISBN: 978-1-4939-0326-9
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