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Protocols for Investigating the Microbial Communities of Oil and Gas Reservoirs

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Recent studies have shown that microorganisms and microbial activity in oil reservoirs and the associated production systems are much more prominent than was originally thought. These findings, in conjunction with technological advances in bio-related disciplines, have revolutionized the way we understand and manage these biological processes.

Here we present a series of protocols outlining the best practices for handling core and produced fluid material from petroleum reservoirs for isolation of nucleic acids, microbial profiling, and whole metagenome sequencing.

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Author information

Authors and Affiliations

  1. Shell International Exploration and Production, Inc., Houston, Texas, USA

    Nicolas Tsesmetzis

  2. School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne, UK

    Michael J. Maguire & Ian M. Head

  3. Shell Global Solutions International B.V.,, Rijswijk, Netherlands

    Bart P. Lomans

Authors
  1. Nicolas Tsesmetzis
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  2. Michael J. Maguire
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  3. Ian M. Head
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  4. Bart P. Lomans
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Corresponding author

Correspondence to Ian M. Head .

Editor information

Editors and Affiliations

  1. University of Essex, School of Biological Sciences University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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Tsesmetzis, N., Maguire, M.J., Head, I.M., Lomans, B.P. (2016). Protocols for Investigating the Microbial Communities of Oil and Gas Reservoirs. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2016_212

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  • DOI: https://doi.org/10.1007/8623_2016_212

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-53116-7

  • Online ISBN: 978-3-662-53118-1

  • eBook Packages: Springer Protocols

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