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Part of the series Methods in Molecular Biology pp 1-11

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Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila

  • Tsuyoshi InoshitaAffiliated withDepartment of Treatment and Research in Multiple Sclerosis and Neuro-Intractable Disease, Juntendo University Graduate School of Medicine
  • , Kahori Shiba-FukushimaAffiliated withDepartment of Treatment and Research in Multiple Sclerosis and Neuro-Intractable Disease, Juntendo University Graduate School of Medicine
  • , Hongrui MengAffiliated withResearch Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine
  • , Nobutaka HattoriAffiliated withDepartment of Treatment and Research in Multiple Sclerosis and Neuro-Intractable Disease, Juntendo University Graduate School of MedicineDepartment of Research for Parkinson’s Disease, Juntendo University Graduate School of MedicineDepartment of Neurology, Juntendo University Graduate School of Medicine
  • , Yuzuru ImaiAffiliated withDepartment of Research for Parkinson’s Disease, Juntendo University Graduate School of MedicineDepartment of Neurology, Juntendo University Graduate School of Medicine Email author 

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Abstract

Mitochondrial quality control is a key process in tissues with high energy demands, such as the brain and muscles. Recent studies using Drosophila have revealed that the genes responsible for familial forms of juvenile Parkinson’s disease (PD), PINK1 and Parkin regulate mitochondrial function and motility. Cell biological analysis using mammalian cultured cells suggests that the dysregulation of mitophagy by PINK1 and Parkin leads to neurodegeneration in PD. In this chapter, we describe the methods to monitor mitochondrial morphology in the indirect flight muscles of adult Drosophila and Drosophila primary cultured neurons and the methods to analyze the motility of mitochondria in the axonal transport of living larval motor neurons.

Keywords:

Axonal transport Fluorescence imaging Mitochondrial fusion and fission Muscle mitochondria Primary neuron culture