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Part of the series Methods in Molecular Biology pp 1-9

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Induction of PINK1/Parkin-Mediated Mitophagy

  • Shigeto SatoAffiliated withDepartment of Neurology, Juntendo University Graduate School of Medicine
  • , Norihiko FuruyaAffiliated withDepartment of Neurology, Juntendo University Graduate School of MedicineDepartment of Neuroscience for Neurodegenerative Disorders, Juntendo University Graduate School of Medicine Email author 

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Abstract

PINK1/Parkin mitophagy is a key mechanism to contribute mitochondrial quality control, and the defects are thought to be a cause of those Parkinson’s disease onsets. Upon loss of mitochondrial membrane potential, PINK1 and Parkin are activated to promote the proteasomal degradation of mitochondrial outer membrane proteins and selective elimination of damaged mitochondria by autophagy. In this chapter, we describe the methods for induction of PINK1/Parkin-mediated mitophagy in tissue culture cell lines.

Keywords:

Mitochondrial membrane potential Parkin PINK1