Protocol

Part of the series Methods in Molecular Biology pp 1-15

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A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule

  • Jun-ichi MorishigeAffiliated withDepartment of Cellular and Molecular Function Analysis, Kanazawa University Graduate School of Medical Science
  • , Ryouhei YamashitaAffiliated withInstitute of Biomedical Sciences, Tokushima University Graduate School
  • , Tamotsu TanakaAffiliated withInstitute of Biomedical Sciences, Tokushima University Graduate School Email author 
  • , Kiyoshi SatouchiAffiliated withDepartment of Applied Biological Science, Fukuyama University

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Abstract

Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.

Keywords:

Ceramide-1-phosphate Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Phos-tag Sphingosine-1-phosphate