Abstract
Flow cytometry is a powerful tool for phenotypic and functional analyses of single immune cells. The increasing capability of flow cytometry technology has driven a more detailed understanding of immune cell subsets and functions in complex cellular systems such as the developing human decidua/placenta. We propose a standardized procedure for the isolation and analysis of human decidual natural killer (dNK) cells and this method can be extended to investigation of other uterine lymphocytes. Here this platform is used to examine the expression of sphingosine-1-phosphate (S1P) receptor and functional growth factors by dNK cells.
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Acknowledgement
This study was supported by Canadian Institutes of Health Research (CIHR) grants (MOP82811, MOP130550, and FDN-143262) to Dr. S. J. Lye. We thank Dr. B. Anne Croy (Queen’s University) for her critical review and helpful advice. We thank the donors, the Research Centre for Women’s and Infants’ Health BioBank Program of Lunenfeld-Tanenbaum Research Institute (LTRI) and the Sinai Health System/University Health Network (Toronto, Canada) for providing human specimens.
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Zhang, J., Bang, A., Lye, S.J. (2017). Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-parametric Flow Cytometry. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 1697. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_24
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DOI: https://doi.org/10.1007/7651_2017_24
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Publisher Name: Humana Press, New York, NY
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