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Part of the series Methods in Molecular Biology pp 1-11

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Exploring MicroRNAs on NIX-Dependent Mitophagy

  • Wen LiAffiliated withInstitute of Neurology, Guangdong Key Laboratory of Age-related Cardiac-cerebral Vascular Disease, The Affiliated Hospital of Guangdong Medical University, Guangdong Medical University
  • , Hao ChenAffiliated withInstitute of Neurology, Guangdong Key Laboratory of Age-related Cardiac-cerebral Vascular Disease, The Affiliated Hospital of Guangdong Medical University, Guangdong Medical University
  • , Shupeng LiAffiliated withInstitute of Neurology, Guangdong Key Laboratory of Age-related Cardiac-cerebral Vascular Disease, The Affiliated Hospital of Guangdong Medical University, Guangdong Medical University
  • , Guanghong LinAffiliated withInstitute of Neurology, Guangdong Key Laboratory of Age-related Cardiac-cerebral Vascular Disease, The Affiliated Hospital of Guangdong Medical University, Guangdong Medical University
  • , Du FengAffiliated withInstitute of Neurology, Guangdong Key Laboratory of Age-related Cardiac-cerebral Vascular Disease, The Affiliated Hospital of Guangdong Medical University, Guangdong Medical UniversityThe Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Medical School Email author 

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Abstract

The dysregulation of autophagy is implicated in many pathological disorders including infections, aging, neurodegenerative diseases, and cancer. Autophagy can be precisely controlled both transcriptionally and translationally. Accumulating evidences show that the autophagy response is regulated by microRNAs, which therefore becomes subject area of interest in recent years. Herein, we give a brief introduction of the recent advancement in the regulation of microRNA on autophagy, and then we focus on the microRNA regulation of the mitophagy receptor, NIX. Finally, we present the methodology on how to study it in detail.

Keywords:

MicroRNAs NIX BNIP3L Autophagy Mitophagy LC3