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Part of the series Methods in Molecular Biology pp 1-10

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Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants

  • Kentaro FurukawaAffiliated withDepartment of Cellular Physiology, Niigata University Graduate School of Medical and Dental Sciences
  • , Tomotake KankiAffiliated withDepartment of Cellular Physiology, Niigata University Graduate School of Medical and Dental Sciences Email author 

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Abstract

Mitochondrial autophagy (mitophagy) is a process that selectively degrades mitochondria via autophagy. Recent studies have shown that mitophagy plays an important role in mitochondrial homeostasis by degrading damaged or excess mitochondria. The budding yeast Saccharomyces cerevisiae is a powerful model organism that has been employed to study several biological phenomena. Recently, there has been significant progress in the understanding of mitophagy in yeast following the identification of Atg32, a mitochondrial outer membrane receptor protein for mitophagy. In this chapter, we describe protocols to study mitophagy in yeast via a genome-wide screen for mitophagy-deficient mutants using fluorescence microscopy and immunoblotting.

Keywords:

Yeast Mitochondria Mitophagy Genome-wide screening Fluorescence microscopy Immunoblotting