Abstract
Aberrant mitochondrial function is associated with many neurological diseases. Mitophagy is a key mechanism for the elimination of damaged mitochondria and maintenance of mitochondrial homeostasis. Induced pluripotent stem (iPS) cell technologies developed over the last decade have allowed us to analyze functions of the human neuron. Here we describe an efficient induction method from human iPS cells to neurons, followed by an image-based mitophagy assay.
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Acknowledgments
This work was supported by JSPS KAKENHI Grant Number JP16K19524 to K.I.; the Project for the Realization of Regenerative Medicine and Support for Core Institutes for iPS Cell Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to H.O.; Research Center Network for Realization Research Centers/Projects of Regenerative Medicine (the Program for Intractable Disease Research utilizing disease-specific iPS Cells) from the Japan Science and Technology Agency (JST) and Japan Agency for Medical Research and Development (AMED) to H.O.; the New Energy and Industrial Technology Development Organization (NEDO) to H.O. and W.A.; the Japan Society for the Promotion of Science (JSPS) to W.A.; and a Grant-in-Aid for the Global COE Program from MEXT to Keio University. H.O. is a scientific consultant for SanBio, Co. Ltd., Eisai, Co., Ltd.
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Ishikawa, KI., Yamaguchi, A., Okano, H., Akamatsu, W. (2017). Assessment of Mitophagy in iPS Cell-Derived Neurons. In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_10
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DOI: https://doi.org/10.1007/7651_2017_10
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