Protocol

Part of the series Methods in Molecular Biology pp 1-19

Date:

Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections

  • Winnie Y. ZouAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Sarah E. BluttAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Sue E. CrawfordAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Khalil EttayebiAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Xi-Lei ZengAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Kapil SaxenaAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Sasirekha RamaniAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Umesh C. KarandikarAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of Medicine
  • , Nicholas C. ZachosAffiliated withDivision of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine
    • , Mary K. EstesAffiliated withDepartment of Molecular Virology and Microbiology, Baylor College of MedicineDepartment of Medicine, Baylor College of Medicine Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Human rotavirus (HRV) and human norovirus (HuNoV) infections are recognized as the most common causes of epidemic and sporadic cases of gastroenteritis worldwide. The study of these two human gastrointestinal viruses is important for understanding basic virus-host interactions and mechanisms of pathogenesis and to establish models to evaluate vaccines and treatments. Despite the introduction of live-attenuated vaccines to prevent life-threatening HRV-induced disease, the burden of HRV illness remains significant in low-income and less-industrialized countries, and small animal models or ex vivo models to study HRV infections efficiently are lacking. Similarly, HuNoVs remained non-cultivatable until recently. With the advent of non-transformed human intestinal enteroid (HIE) cultures, we are now able to culture and study both clinically relevant HRV and HuNoV in a biologically relevant human system. Methods described here will allow investigators to use these new culture techniques to grow HRV and HuNoV and analyze new aspects of virus replication and pathogenesis.

Keywords

Gastrointestinal viral infections HIEs Human intestinal enteroids Human norovirus Human rotavirus