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Effect of transforming growth factor-beta 1 (TGF-ß1) released from a scaffold on chondrogenesis in an osteochondral defect model in the rabbit

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Articular cartilage repair might be stimulated by the controlled delivery of therapeutic factors. We tested the hypotheses whether TGF-ß1 can be released from a polymeric scaffold over a prolonged period of time in vitro and whether its transplantation modulates cartilage repair in vivo. Unloaded control or TGF-ß1 poly(ether-ester) copolymeric scaffolds were applied to osteochondral defects in the knee joints of rabbits. In vitro, a cumulative dose of 9 ng TGF-ß1 was released over 4 weeks. In vivo, there were no adverse effects on the synovial membrane. Defects treated with TGF-ß1 scaffolds showed no significant difference in individual parameters of chondrogenesis and in the average cartilage repair score after 3 weeks. There was a trend towards a smaller area (42.5 %) of the repair tissue that stained positive for safranin O in defects receiving TGF-ß1 scaffolds. The data indicate that TGF-ß1 is released from emulsion-coated scaffolds over a prolonged period of time in vitro and that application of these scaffolds does not significantly modulate cartilage repair after 3 weeks in vivo. Future studies need to address the importance of TGF-ß1 dose and release rate to modulate chondrogenesis.

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Authors and Affiliations

  1. Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany

    Magali Cucchiarini, Karin Mitosch, Gunter Kaul, Dieter Kohn & Henning Madry

  2. OctoPlus, Zernikedreef 12, 2333 CL, Leiden, The Netherlands

    Jerome Sohier & Jeroen M. Bezemer

  3. Departments of Biostatistics and Orthopaedic Surgery, Children’s Hospital, Harvard Medical School, Boston, Massachusetts, USA

    David Zurakowski

  4. Amgen, One Amgen Center Drive, Thousand Oaks, CA, 91320, USA

    Jeroen M. Bezemer

Authors
  1. Magali Cucchiarini
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  2. Jerome Sohier
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  3. Karin Mitosch
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  4. Gunter Kaul
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  6. Jeroen M. Bezemer
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  8. Henning Madry
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Cucchiarini, M., Sohier, J., Mitosch, K. et al. Effect of transforming growth factor-beta 1 (TGF-ß1) released from a scaffold on chondrogenesis in an osteochondral defect model in the rabbit. cent.eur.j.biol. 1, 43–60 (2006). https://doi.org/10.2478/s11535-006-0004-8

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  • DOI: https://doi.org/10.2478/s11535-006-0004-8

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